产品货号：
Z11381
中文名称：
ATG14抗体
英文名称：
Anti-ATG14 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-ATG14 Antibody |
| 指标别名 | ATG14;Atg14L;Barkor;KIAA0831 |
| 克隆性 | Polyclonal |
| 检验物种 | human,rat |
| 应用范围 | WB,IHC,ICC/IF,IF,FCM |
| 基因名称 | ATG14 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human ATG14L (70-101aa RDRERFIDKKERLSRLKSKQEEFQKEVLKAME),different from the related mouse and rat sequences by two amino acids. |
| 实际分子量 | 59KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | ATG14 (also known as beclin-1-associated autophagy-related key regulator (Barkor) or ATG14L),an essential autophagy-specific regulator of the class III phosphatidylinositol 3-kinase complex,promotes membrane tethering of protein-free liposomes,and enhances hemifusion and full fusion of proteoliposomes reconstituted with the target (t)-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) syntaxin 17 (STX17) and SNAP29,and the vesicle (v)-SNARE VAMP8 (vesicle-associated membrane protein 8).ATG14 binds to the SNARE core domain of STX17 through its coiled-coil domain,and stabilizes the STX17-SNAP29 binary t-SNARE complex on autophagosomes. |
| Uniprot ID | Q6ZNE5 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | autophagy related 14 |
| 蛋白名全称 | Beclin 1-associated autophagy-related key regulator |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunofluorescence (IF) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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